Molecular Genetics¶
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3 domain-specific abstractions whose origin domain is Molecular Genetics.
- Palindromic Sequence — A DNA or RNA sequence invariant under reverse complementation, so either antiparallel strand read in the same direction has the same nucleotide order.
- Surveyor Nuclease Assay — A targeted mismatch-cleavage assay that PCR-amplifies a locus, denatures and reanneals sequence variants into heteroduplexes, uses Surveyor nuclease to cut beside mismatches, and reads fragment sizes and intensities as evidence of variants and approximate editing frequency.
- Three-Prime Untranslated Region — The transcript-specific segment after a protein-coding region's termination codon and before the mature RNA's 3′ end, where sequence, structure, and bound factors can govern cleavage, stability, localization, and translation without changing the encoded polypeptide.