A Viability Quantitative PCR Dilemma

Holm, W. V., Ghesquière, J., Boon, N., Verspecht, T., Bernaerts, K., Zayed, N., Chatzigiannidou, I., et al. (2021). A Viability Quantitative PCR Dilemma: Are Longer Amplicons Better?. Applied and Environmental Microbiology.

Type

Journal article

Intellectual base

Primary research

Year

2021

DOI

10.1128/aem.02653-20

Link

https://doi.org/10.1128/AEM.02653-20

Cited by

1 citation across 1 artifact.

Domain-specific

• Viability PCR
  • A multi-species experiment found improved discrimination as amplicons lengthened into roughly the first few hundred base pairs, followed by diminishing returns and efficiency costs; this is evidence for a design trade-off, not a universal optimal length.

Verification

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Links previously used in the corpus

Before the registry existed this work was also linked 1 other way.

• https://pubmed.ncbi.nlm.nih.gov/33361365/ ×1

Registry ID ref:bc89801eb187 · see in the full table