Specific Enzymatic Amplification of DNA In Vitro¶
Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., & Erlich, H. (1986). Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction. Cold Spring Harbor Symposia on Quantitative Biology, 51, 263-273.
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Primes¶
- Complementarity
- Because the complement is uniquely fit and computationally specified by the matching rule, the same structure is deliberately reused in PCR primer design (synthesize the exact complement of a target region to bind it)
This sourceDescribes PCR, in which primers are synthesized as the exact complement of a target region to bind it. (
- Because the complement is uniquely fit and computationally specified by the matching rule, the same structure is deliberately reused in PCR primer design (synthesize the exact complement of a target region to bind it)
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